c reactive protein crp levels  (Abbott Laboratories)

Journal: Journal of Inflammation (London, England)

Article Title: C-reactive protein modulates lipid mediators in a pro-inflammatory direction

doi: 10.1186/s12950-025-00459-5

Figure Lengend Snippet: Modulation of inflammatory cytokines by CRP in vivo and in vitro ( A–D ) Ten-week-old C57/BL6 mice were injected with an adenovirus vector encoding human CRP (CRP) or a control blank adenovirus vector (Null). Five days after the viral injection, modulation analyses were performed ( n = 6/group). Shown are ( A ) The CRP levels of the plasma collected from the mice administered with adenovirus coding CRP or blank adenovirus (Null), as well as recombinant CRP described in the Method section , were determined by Western blotting. We have the western blots twice and this panel is the representative of the totality of all the western blotting results. The bands which were deemed, based on the molecular weights, as CRP were marked with arrows. ( B ) Plasma CRP levels as determined by ELISA. ( C ) Plasma TNF-a levels. ( D ) Plasma IL-6 levels. ( E-H ) RAW264.7 cells treated with recombinant CRP at concentrations of 0, 1, or 10 mg/dL ( E, F ) or conditioned medium of HepG2 cells infected with Ad-CRP or Ad-Null (i.e., CM-CRP or CM-Null) or with a mixture of CM-CRP and CM-Null at the ratio of 1:9. These treatments were prepared as described in the Materials and Methods section ( G, H ) ( n = 6). After 24 h, the TNF-a ( E, G ) and IL-6 ( F, H ) levels of the medium were determined via ELISA and relative protein values were adjusted to cellular protein levels. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human CRP levels were measured using a DCRP00B ELISA kit (R&D Systems, Inc., MN, USA).

Techniques: In Vivo, In Vitro, Injection, Plasmid Preparation, Control, Clinical Proteomics, Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay, Infection

Journal: Journal of Inflammation (London, England)

Article Title: C-reactive protein modulates lipid mediators in a pro-inflammatory direction

doi: 10.1186/s12950-025-00459-5

Figure Lengend Snippet: Modulation of cellular and medium lipid mediators in CRP-overexpressing HepG2 cells. HepG2 cells were infected with an adenovirus vector encoding human CRP (CRP) or a blank adenovirus vector (Null) ( n = 4). After 48 h, the culture medium was replaced with a serum-free medium. After a further 24 h, the cellular components (i.e., A–G, O–U ) and the medium (i.e., H–N, V, W ) were collected for analyses involving lysophospholipids ( A–N ), eicosanoids, and related mediators ( O–W ). ns: not significant, * P < 0.05

Article Snippet: Human CRP levels were measured using a DCRP00B ELISA kit (R&D Systems, Inc., MN, USA).

Techniques: Infection, Plasmid Preparation